Laboratory for Multi-Scale Imaging

Location : Burchard building B-013

• Enable high performance and resolution SEM imaging

• Can work with various type of “dried” samples

• Live quantitative EDS system embedded with ChemiSEM platform

• 1 nm image resolution depends on samples/imaging conditions

• Can run from 20 eV up to 30 keV electron energy

• Biased stage up to 4 kV bias voltage

• ETD, T1, T2, T3, and retractable BS detectors

See more details on the vendor’s website:

https://www.thermofisher.com/us/en/home/electron-microscopy/products/scanning-electron-microscopes/apreo-sem.html#features

Location: Burchard building B-012

• High resolution 3D x-ray microscope

• 40 to 100 kV x-ray source. < 5um spot size at 4W power. 10W max. power

• 16 MP sCMOS (4096x4096 pixels) detector

• Sample size is up to 80 mm height and 75 mm in diameter

• Build in software for:

  • NRECON – Transforms the 2D projection images into 3D volumes

  • DATAVIEWER – Slice-by-slice inspection of 3D volumes and 2D/3D image registration

  • CTVOX – Realistic visualization by volume rendering

  • CTAN – 2D/3D image analysis & processing

  • CTVOL – Visualization of surface models to export for CAD or 3D printing

See more details on the vendor’s website:

https://www.bruker.com/en/products-and-solutions/diffractometers-and-x-ray-microscopes/3d-x-ray-microscopes/skyscan-1272.html?utm_term=Wp%20SKYSCAN%201272%20CMOS%20Edition&utm_campaign=Bruker%20FIRST%20Newsletter%202023%20Jun%20-%20Benchtops%3A%20Small%20Size%2C%20Big%20Impact&utm_content=email&utm_source=Act-On+Software&utm_medium=email&cm_mmc=Act-On%20Software-_-email-_-Bruker%20FIRST%20Newsletter%202023%20Jun%20-%20Benchtops%3A%20Small%20Size%2C%20Big%20Impact-_-Wp%20SKYSCAN%201272%20CMOS%20Edition

Location: Burchard building B-011A

• Fully automatic microscope controlled via NIS Elements software

• Noise piece focusing

• High speed and sensitivity BT Fusion sCMOS camera

• 4 color LEDs for DAPI, Cy3, TexasRed, and Cy5 florescent imaging

• Integrated with high-speed AX confocal that has 4 color lasers – UV, Blue, Green, and Red

See more details on the vendor’s website:

https://www.microscope.healthcare.nikon.com/products/upright-microscopes/eclipse-ni-series

https://www.microscope.healthcare.nikon.com/products/confocal-microscopes/ax

https://www.microscope.healthcare.nikon.com/products/software/nis-elements

Spec of the 2100Plus at Stevens

• 200kV LaB6 filament

• High-specimen tilt (HT) pole piece

• Point resolution @ 0.25 nm; Lattice resolution at 0.14 nm

• HT can be selected at 80, 100, 120, 160, and 200 kV

• Objective lens

  • Focus distance at 2.7 mm

  • Spherical aberration at 1.4 mm

  • Chromatic aberration at 1.8 mm

  • Minimum focus step at 1.8 nm

• Spot size (in diameter)

  • TEM mode: 20 to 200 nm

  • EDS, NBD, CBD mode: 1.5 to 25 nm

• Stage

  • X, Y: +/- 1.0 mm

  • Z: +/- 0.2 mm

• Stage Tilt

  • With regular holder: +/- 42 degree in alpha and +/- 30 degree in beta tilt

  • With high-tilt holder: +/- 80 degree in alpha tilt only

• Image shift: +/- 2.0 um

• Florescent Screen

  • Large screen: 160 mm in diameter

  • Small screen: 25mm in diameter

• Available holders:

  • Single tilt holder

  • Double tilt low background holder

  • Single tilt high-angle tiling holder

  • Gatan ELSA single tilt high-angle tilting cryo-holder

• Capabilities

  • Digital imaging via a Gatan 4k by 4k Rio camera

  • BF/DF TEM imaging

  • BF/DF STEM imaging

  • Electron diffraction

  • TEM tomography

  • Cryo-microscopy

  • EDS is not available now but will be coming soon!

Regarding to basic information of becoming a self-user, hourly rate, training and etc., please click here to download the LMSI TEM Users Guide. Please contact Dr. Tsengming (Alex) Chou at 201-216-8310 if you have any questions.

Nanotechnology Workstation : combine both FE-SEM and HR-FIB, sample can be manipulated in nano-scale.

High Resolution FESEM
a GEMINI field-emission electron column that provides resolution of 1.0 nm @ 15 kV and 1.9 nm @ 1 kV.

This FE-SEM can:
magnification: 12x to 1000 kx
probe current: 4pA to 20 nA
HV: 0.1 to 30 kV
with in-lens SE detector, Everhart-Thornley type SE chamber detector, in-lens EsB (energy selective backscattering) detector, and a STEM BF/DF detector for the thin specimen.
N2 gas charge compensation device is also available.

High Resolution FIB
Resolution down to 2.5 nm @ 30 kV via this Cobra ion column
source: liquid metal gallium ion source
magnification: 300x to 500kx
probe current: 1 pA to 50 nA
HV: 1.0 to 30 kV
with Pt GIS (gas injection system) to provide both electron and ion beam induced deposition.

Introduction to 3D Reconstruction
Image stack of serial sectioned images at nano-scale can be produced on this system. Using Avizo 3D reconstruction and visualization platform, studies of 3D nano-structure can be achieved.

High Resolution STEM Imaging
Using the retractable BF/DF STEM detector, sample can be imaged in STEM (scanning transmission electron microscopy) mode via electrons have up to 30 kV energy. It provides structure information on a thin specimen.

TEM Sample Preparation
Automatic TEM sample preparation can be done using in-situ lift out method via Kleindiek nano-manipulator (model: MM3A).

Advanced Analytical Platform
A 80 mm2 Oxford SDD (silicon drift detector) EDS system provides a high through-put and high resolution microanalysis capability.

Electron and Ion Beam Lithography
Via Nabity's NPGS system, lithography can be done via either electron or ion beam.

Cryo-capability
A Leica VCT cryo-transfer system is coming soon to provide a cryo-EM capability on this system.

BioScope Resolve's seamless integration of atomic force microscopy and light microscopy reveals more detail and information of biological samples including mechanical properties. The system's unique design makes combined optical and AFM research easy with its open access to the sample, single instrument setup, and correlated data collection and analysis. Researchers can now perfectly connect real-time optical images with AFM imaging.

• The new MIROView graphical interface and ScanAsyst-Cell mode ensure expert data generation, regardless of the user’s AFM experience level.

• Integrates with a Nikon Ti-2 Florescent microscope with:

  • X-cite 120LED Boost light engine

  • 5 filter cubes - BF, DAPI, GFP, DesRed, Cy5

  • 4 Obj lens - 4x, 10x, ELWD20x , and ELWD40x 

  • DIC and BF imaging

• Single, integrated view and operation controls for AFM or optical microscope

• Syncronized AFM images, force maps and single force curves with optical images and data

• Point-and-click setup for automated force and imaging measurements

• Video creation of experiments showing optical and AFM results

• BioAFM accessories and PeakForce Tapping probes to meet every application

• PeakForceQNM mode for accurate mechanical measurement of the materials

Regarding basic information on becoming a self-user, hourly rate, training, etc., please contact Dr. Tsengming (Alex) Chou at tchou@stevens.edu if you have any questions.

Nikon E-1000 up-right florescent microscope with automatic stage control

A high sensitive monochromatic CCD camera for florescent imaging

Nikon C-1 LSCM with 3 lasers

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Kakanuru, P., & Pochiraju, K. (2022). Simulation of shrinkage during sintering of additively manufactured silica green bodies. Additive Manufacturing, 56. https://doi.org/10.1016/j.addma.2022.102908

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Mir, S. M., Chen, J., Pinezich, M. R., O’Neill, J. D., Huang, S. X. L., Vunjak-Novakovic, G., & Kim, J. (2022). Imaging-guided bioreactor for de-epithelialization and long-term cultivation of ex vivo rat trachea. Lab on a Chip, 22(5), 1018–1031. https://doi.org/10.1039/d1lc01105g

Na Nagara, V., Sarkar, D., Elzinga, E. J., & Datta, R. (2022). Removal of heavy metals from stormwater runoff using granulated drinking water treatment residuals. Environmental Technology and Innovation, 28. https://doi.org/10.1016/j.eti.2022.102636

Robbins, J. P., Ezeonu, L., Tang, Z., Yang, X., Koel, B. E., & Podkolzin, S. G. (2022). Propane Dehydrogenation to Propylene and Propylene Adsorption on Ni and Ni-Sn Catalysts. ChemCatChem. https://doi.org/10.1002/cctc.202101546

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Sul, O., Seo, H., Choi, E., Kim, S., Gong, J., Bang, J., Ju, H., Oh, S., Lee, Y., Sun, H., Kwon, M., Kang, K., Hong, J., Yang, E.-H., Chung, Y., & Lee, S.-B. (2022). An Ultralow Power Mixed Dimensional Heterojunction Transistor Based on the Charge Plasma pn Junction. Small. https://doi.org/10.1002/smll.202202153

Sun, J., Wang, J., Hu, W., Wang, Y., Zhang, Q., Hu, X., Chou, T., Zhang, B., Gallaro, C., Halloran, M., Liang, L., Ren, L., & Wang, H. (2022). A Porous Bimetallic Au@Pt Core-Shell Oxygen Generator to Enhance Hypoxia-Dampened Tumor Chemotherapy Synergized with NIR-II Photothermal Therapy. ACS Nano. https://doi.org/10.1021/acsnano.2c02528

Tang, R., & Du, H. (2022). Fabrication of anodic aluminum oxide on silica optical fiber with nanopore channels oriented off surface normal. Journal of the American Ceramic Society. https://doi.org/10.1111/jace.18621

Wang, H., Zhou, X., Wang, J., Zhang, X., Zhu, M., & Wang, H. (2022). Fabrication of channeled scaffolds through polyelectrolyte complex (PEC) printed sacrificial templates for tissue formation. Bioactive Materials. https://doi.org/10.1016/j.bioactmat.2022.01.030

Wu, D., Ge, Y., Li, R., Feng, Y., & Akcora, P. (2022). Thermally Activated Shear Stiffening in Polymer-Grafted Nanoparticle Composites for High-Temperature Adhesives. ACS Applied Polymer Materials. https://doi.org/10.1021/acsapm.2c00097

Zhang, F., Cao, K., Zaeri, A., Zgeib, R., & Chang, R. C. (2022a). Design, fabrication, and analysis of spatially heterogeneous scaffold by melt electrospinning writing of poly(ε-Caprolactone). Journal of Applied Polymer Science. https://doi.org/10.1002/app.52235

Zhang, F., Cao, K., Zaeri, A., Zgeib, R., & Chang, R. C. (2022b). Effects of Printing Sequence on the Printing Accuracy of Melt Electrowriting Scaffolds. Macromolecular Materials and Engineering. https://doi.org/10.1002/mame.202200222

Zhang, F., Cao, K., Zaeri, A., Zgeib, R., & Chang, R. C. (2022c). Effects of scaffold design parameters on the printing accuracy for melt electrowriting. Journal of Manufacturing Processes, 81, 177–190. https://doi.org/10.1016/j.jmapro.2022.06.070

Zhao, W., Libera, M., Prysak, M., Katz, J., & De Stefano, L. (2022). An in vitro model of microbial contamination in the operating room. Journal of Biomedical Materials Research - Part B Applied Biomaterials. https://doi.org/10.1002/jbm.b.35104

Asawa, K., Kumar, S., Huang, Y., & Choi, C. H. (2021). Guiding light via slippery liquid-infused porous surfaces. Applied Physics Letters, 118(9), 091602. https://doi.org/10.1063/5.0038910

Chen, J., Mir, S. M., Pinezich, M. R., O’Neill, J. D., Guenthart, B. A., Bacchetta, M., Vunjak-Novakovic, G., Huang, S. X. L., & Kim, J. (2021). Homogeneous Distribution of Exogenous Cells onto De-epithelialized Rat Trachea via Instillation of Cell-Loaded Hydrogel. ACS Biomaterials Science & Engineering, acsbiomaterials.1c01031. https://doi.org/10.1021/ACSBIOMATERIALS.1C01031

Liang, J., Xiao, X., Chou, T.-M., & Libera, M. (2021). Analytical Cryo-Scanning Electron Microscopy of Hydrated Polymers and Microgels. Accounts of Chemical Research, 54(10), 2386–2396. https://doi.org/10.1021/acs.accounts.1c00109

Liu, Y., & Choi, C.-H. (2021). Superhydrophobic Sands for the Preservation and Purification of Water. Coatings, 11(2), 151. https://doi.org/10.3390/coatings11020151

Sun, J., Wang, J., Hu, W., Wang, Y., Chou, T., Zhang, Q., Zhang, B., Yu, Z., Yang, Y., Ren, L., & Wang, H. (2021). Camouflaged Gold Nanodendrites Enable Synergistic Photodynamic Therapy and NIR Biowindow II Photothermal Therapy and Multimodal Imaging. ACS Applied Materials & Interfaces, 13(9), 10778–10795. https://doi.org/10.1021/acsami.1c01238

Tang, Z., Chen, T., Liu, K., Du, H., & Podkolzin, S. G. (2021). Atomic, Molecular and Hybrid Oxygen Structures on Silver. Langmuir, 37(39), 11603–11610. https://doi.org/10.1021/ACS.LANGMUIR.1C01941

Wei, J., Duan, L., Wei, J., Hoffmann, E., Song, Y., & Meng, X. (2021). Lead removal from water using organic acrylic amine fiber (AAF) and inorganic-organic P-AAF, fixed bed filtration and surface-induced precipitation. Journal of Environmental Sciences (China), 101, 135–144. https://doi.org/10.1016/j.jes.2020.08.009

Xiao, X., Ji, J., Zhao, W., Nangia, S., & Libera, M. (2021). Salt Destabilization of Cationic Colistin Complexation within Polyanionic Microgels. Macromolecules. https://doi.org/10.1021/acs.macromol.1c02157

Chang, T. L., Sun, P. K., Zhou, X., Besser, R. S., & Liang, J. (2020). Preparation and electrochemical performances of silver (alloy) nanoparticles decorated on reduced graphene oxide, using self-polymerization of dopamine in an acidic environment. Materials Today Chemistry, 17, 100312. https://doi.org/10.1016/j.mtchem.2020.100312

Chang, W., Shah, M. B., Zhou, G., Walsh, K., Rudraiah, S., Kumbar, S. G., & Yu, X. (2020). Polymeric nanofibrous nerve conduits coupled with laminin for peripheral nerve regeneration. Biomedical Materials (Bristol), 15(3). https://doi.org/10.1088/1748-605X/ab6994

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Hu, W., Song, X., Yu, H., Sun, J., & Zhao, Y. (2020). Released Exosomes Contribute to the Immune Modulation of Cord Blood-Derived Stem Cells. Frontiers in Immunology, 11. https://doi.org/10.3389/fimmu.2020.00165

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Junka, R., & Yu, X. (2020). Polymeric nanofibrous scaffolds laden with cell-derived extracellular matrix for bone regeneration. Materials Science and Engineering C, 113. https://doi.org/10.1016/j.msec.2020.110981

Kalyon, D. M., & Fisher, F. T. (2020). Impact of ultrasonication on carbon nanotube demixing and damage in polymer nanocomposites. Journal of Applied Polymer Science. https://doi.org/10.1002/app.49370

Kumar, A., Mir, S. M., Aldulijan, I., Mahajan, A., Anwar, A., Leon, C. H., … Yu, X. (2020). Load-bearing biodegradable PCL-PGA-beta TCP scaffolds for bone tissue regeneration. Journal of Biomedical Materials Research - Part B Applied Biomaterials. https://doi.org/10.1002/jbm.b.34691

Shi, Q., Zhang, S., Ge, J., Wei, J., Christodoulatos, C., Korfiatis, G. P., & Meng, X. (2020). Lead immobilization by phosphate in the presence of iron oxides: Adsorption versus precipitation. Water Research, 179, 115853. https://doi.org/10.1016/j.watres.2020.115853

Wang, J., Sun, J., Hu, W., Wang, Y., Chou, T., Zhang, B., … Wang, H. (2020). A Porous Au@RhBimetallic Core–Shell Nanostructure as an H2O2-Driven Oxygenerator to Alleviate Tumor Hypoxia for Simultaneous Bimodal Imaging and Enhanced Photodynamic Therapy. Advanced Materials. https://doi.org/10.1002/adma.202001862

Wang, J., Sun, J., Wang, Y., Chou, T., Zhang, Q., Zhang, B., … Wang, H. (2020). Gold Nanoframeworks with Mesopores for Raman–Photoacoustic Imaging and Photo-Chemo Tumor Therapy in the Second Near-Infrared Biowindow. Advanced Functional Materials. https://doi.org/10.1002/adfm.201908825

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Yang, Y., Robbins, J. P., Ezeonu, L., Ma, Y., Sparta, N., Kong, X., … Lee, S. S. (2020). Probing lattice vibrations of stabilized CsPbI3polymorphs: Via low-frequency Raman spectroscopy. Journal of Materials Chemistry C, 8(26), 8896–8903. https://doi.org/10.1039/d0tc02123g

Ye, Z., & Prigiobbe, V. (2020). Transport of produced water through reactive porous media. Water Research, 185, 116258. https://doi.org/10.1016/j.watres.2020.116258

Zhang, S., Shi, Q., Korfiatis, G., Christodoulatos, C., Wang, H., & Meng, X. (2020). Chromate removal by electrospun PVA/PEI nanofibers: Adsorption, reduction, and effects of co-existing ions. Chemical Engineering Journal, 387. https://doi.org/10.1016/j.cej.2020.124179

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Zhou, X., Hou, C., Chang, T. L., Zhang, Q., & Liang, J. F. (2020). Controlled released of drug from doubled-walled PVA hydrogel/PCL microspheres prepared by single needle electrospraying method. Colloids and Surfaces B: Biointerfaces, 187. https://doi.org/10.1016/j.colsurfb.2019.110645

Zong, K., Asawa, K. M., Circelli, A., Sparta, N., Choi, C. H., & Lee, S. S. (2020). Graphoepitaxy-Directed Assembly of Organic Semiconductor Single Crystals into Trellis Structures. ACS Materials Letters, 2(7), 721–726. https://doi.org/10.1021/acsmaterialslett.0c00153

Chang, T.-L., Zhou, X., & Liang, J. (2019). Synthesis and characterization of Ag-Cu alloy nanoparticles for antimicrobial applications: A polydopamine chemistry application. Materials Science and Engineering: C, 98, 675–684. https://doi.org/10.1016/J.MSEC.2018.12.092

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Jiang, Y., Xu, W., Sarshar, M. A., & Choi, C.-H. (2019). Generalized models for advancing and receding contact angles of fakir droplets on pillared and pored surfaces. Journal of Colloid and Interface Science, 552, 359–371. https://doi.org/10.1016/J.JCIS.2019.05.053

Jin, Y., & Lee, W. (2019). Cross-Linking Stabilizes Electrical Resistance of Reduced Graphene Oxide in Humid Environments. Langmuir, 35(16), 5427–5434. https://doi.org/10.1021/acs.langmuir.8b03416

Kong, X., Zong, K., & Lee, S. S. (2019). Nanoconfining Optoelectronic Materials for Enhanced Performance and Stability. Chemistry of Materials, 31(14), 4953–4970. https://doi.org/10.1021/acs.chemmater.9b01707

Kong, X., Shayan, K., Hua, S., Strauf, S., & Lee, S. S. (2019). Complete Suppression of Detrimental Polymorph Transitions in All-Inorganic Perovskites via Nanoconfinement. ACS Applied Energy Materials, 2(4), 2948–2955. https://doi.org/10.1021/acsaem.9b00322

Kumar, A., Zhang, Y., Terracciano, A., Zhao, X., Su, T.-L., Kalyon, D. M., … Yu, X. (2019). Load-bearing biodegradable polycaprolactone-poly (lactic-co-glycolic acid)- beta tri-calcium phosphate scaffolds for bone tissue regeneration. Polymers for Advanced Technologies. https://doi.org/10.1002/pat.4551

Lee, J., Wooh, S., & Choi, C.-H. (2019). Fluorocarbon lubricant impregnated nanoporous oxide for omnicorrosion-resistant stainless steel. Journal of Colloid and Interface Science, 558, 301–309. https://doi.org/10.1016/J.JCIS.2019.09.117

Liang, J., Wang, H., & Libera, M. (2019). Biomaterial surfaces self-defensive against bacteria by contact transfer of antimicrobials. Biomaterials, 204, 25–35. https://doi.org/10.1016/J.BIOMATERIALS.2019.03.006

Liang, J., Xiao, X., Chou, T.-M., & Libera, M. (2019). Counterion Exchange in Peptide-Complexed Core–Shell Microgels. Langmuir, 35(29), 9521–9528. https://doi.org/10.1021/acs.langmuir.9b01058

Liu, K., Ohodnicki, P. R., Kong, X., Lee, S. S., & Du, H. (2019). Plasmonic Au nanorods stabilized within anodic aluminum oxide pore channels against high-temperature treatment. Nanotechnology, 30(40), 405704. https://doi.org/10.1088/1361-6528/ab2a3f

Liu, K., Wuenschell, J., Bera, S., Tang, R., Ohodnicki, P. R., & Du, H. (2019). Nanostructured sapphire optical fiber embedded with Au nanorods for high-temperature plasmonics in harsh environments. Optics Express, 27(26), 38125. https://doi.org/10.1364/OE.27.038125

Liu, S., Liedel, C., Tarakina, N. V., Osti, N. C., & Akcora, P. (2019). Dynamics of ionic liquids in the presence of polymer-grafted nanoparticles. Nanoscale, 11(42), 19832–19841. https://doi.org/10.1039/C9NR04204K

Liu, T., Yang, F., Wang, X., & Liang, J. F. (2019). Adhesive Gold Nanoparticles for Easy and Controlled Surface Coating. Langmuir, 35(7), 2728–2737. https://doi.org/10.1021/acs.langmuir.8b04110

Tourlomousis, F., Jia, C., Karydis, T., Mershin, A., Wang, H., Kalyon, D. M., & Chang, R. C. (2019). Machine learning metrology of cell confinement in melt electrowritten three-dimensional biomaterial substrates. Microsystems & Nanoengineering, 5(1), 15. https://doi.org/10.1038/s41378-019-0055-4

Wang, X., Kang, K., Godin, K., Fu, S., Chen, S., & Yang, E.-H. (2019). Effects of solvents and polymer on photoluminescence of transferred WS 2 monolayers. Journal of Vacuum Science & Technology B, 37(5), 052902. https://doi.org/10.1116/1.5094543

Wei, J., Meng, X., Wen, X., & Song, Y. (2020). Adsorption and recovery of phosphate from water by amine fiber, effects of co-existing ions and column filtration. Journal of Environmental Sciences, 87, 123–132. https://doi.org/10.1016/J.JES.2019.05.009

Yang, F., Chang, T.-L., Liu, T., Wu, D., Du, H., Liang, J., & Tian, F. (2019). Label-free detection of Staphylococcus aureus bacteria using long-period fiber gratings with functional polyelectrolyte coatings. Biosensors and Bioelectronics, 133, 147–153. https://doi.org/10.1016/J.BIOS.2019.03.024

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Ye, Z., Abraham, J., Christodoulatos, C., & Prigiobbe, V. (2019). Mineral Carbonation for Carbon Utilization in Microalgae Culture. Energy & Fuels, 33(9), 8843–8851. https://doi.org/10.1021/acs.energyfuels.9b01232

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