Laboratory for Multi-Scale Imaging

Location : Burchard building B-013

• Enable high performance and resolution SEM imaging

• Can work with various type of “dried” samples

• Live quantitative EDS system embedded with ChemiSEM platform

• 1 nm image resolution depends on samples/imaging conditions

• Can run from 20 eV up to 30 keV electron energy

• Biased stage up to 4 kV bias voltage

• ETD, T1, T2, T3, and retractable BS detectors

See more details on the vendor’s website:

https://www.thermofisher.com/us/en/home/electron-microscopy/products/scanning-electron-microscopes/apreo-sem.html#features

Location: Burchard building B-012

• High resolution 3D x-ray microscope

• 40 to 100 kV x-ray source. < 5um spot size at 4W power. 10W max. power

• 16 MP sCMOS (4096x4096 pixels) detector

• Sample size is up to 80 mm height and 75 mm in diameter

• Build in software for:

  • NRECON – Transforms the 2D projection images into 3D volumes

  • DATAVIEWER – Slice-by-slice inspection of 3D volumes and 2D/3D image registration

  • CTVOX – Realistic visualization by volume rendering

  • CTAN – 2D/3D image analysis & processing

  • CTVOL – Visualization of surface models to export for CAD or 3D printing

See more details on the vendor’s website:

https://www.bruker.com/en/products-and-solutions/diffractometers-and-x-ray-microscopes/3d-x-ray-microscopes/skyscan-1272.html?utm_term=Wp%20SKYSCAN%201272%20CMOS%20Edition&utm_campaign=Bruker%20FIRST%20Newsletter%202023%20Jun%20-%20Benchtops%3A%20Small%20Size%2C%20Big%20Impact&utm_content=email&utm_source=Act-On+Software&utm_medium=email&cm_mmc=Act-On%20Software-_-email-_-Bruker%20FIRST%20Newsletter%202023%20Jun%20-%20Benchtops%3A%20Small%20Size%2C%20Big%20Impact-_-Wp%20SKYSCAN%201272%20CMOS%20Edition

Location: Burchard building B-011A

• Fully automatic microscope controlled via NIS Elements software

• Noise piece focusing

• High speed and sensitivity BT Fusion sCMOS camera

• 4 color LEDs for DAPI, Cy3, TexasRed, and Cy5 florescent imaging

• Integrated with high-speed AX confocal that has 4 color lasers – UV, Blue, Green, and Red

See more details on the vendor’s website:

https://www.microscope.healthcare.nikon.com/products/upright-microscopes/eclipse-ni-series

https://www.microscope.healthcare.nikon.com/products/confocal-microscopes/ax

https://www.microscope.healthcare.nikon.com/products/software/nis-elements

Spec of the 2100Plus at Stevens

• 200kV LaB6 filament

• High-specimen tilt (HT) pole piece

• Point resolution @ 0.25 nm; Lattice resolution at 0.14 nm

• HT can be selected at 80, 100, 120, 160, and 200 kV

• Objective lens

  • Focus distance at 2.7 mm

  • Spherical aberration at 1.4 mm

  • Chromatic aberration at 1.8 mm

  • Minimum focus step at 1.8 nm

• Spot size (in diameter)

  • TEM mode: 20 to 200 nm

  • EDS, NBD, CBD mode: 1.5 to 25 nm

• Stage

  • X, Y: +/- 1.0 mm

  • Z: +/- 0.2 mm

• Stage Tilt

  • With regular holder: +/- 42 degree in alpha and +/- 30 degree in beta tilt

  • With high-tilt holder: +/- 80 degree in alpha tilt only

• Image shift: +/- 2.0 um

• Florescent Screen

  • Large screen: 160 mm in diameter

  • Small screen: 25mm in diameter

• Available holders:

  • Single tilt holder

  • Double tilt low background holder

  • Single tilt high-angle tiling holder

  • Gatan ELSA single tilt high-angle tilting cryo-holder

• Capabilities

  • Digital imaging via a Gatan 4k by 4k Rio camera

  • BF/DF TEM imaging

  • BF/DF STEM imaging

  • Electron diffraction

  • TEM tomography

  • Cryo-microscopy

  • EDS is not available now but will be coming soon!

Regarding to basic information of becoming a self-user, hourly rate, training and etc., please click here to download the LMSI TEM Users Guide. Please contact Dr. Tsengming (Alex) Chou at 201-216-8310 if you have any questions.

Nanotechnology Workstation : combine both FE-SEM and HR-FIB, sample can be manipulated in nano-scale.

High Resolution FESEM
a GEMINI field-emission electron column that provides resolution of 1.0 nm @ 15 kV and 1.9 nm @ 1 kV.

This FE-SEM can:
magnification: 12x to 1000 kx
probe current: 4pA to 20 nA
HV: 0.1 to 30 kV
with in-lens SE detector, Everhart-Thornley type SE chamber detector, in-lens EsB (energy selective backscattering) detector, and a STEM BF/DF detector for the thin specimen.
N2 gas charge compensation device is also available.

High Resolution FIB
Resolution down to 2.5 nm @ 30 kV via this Cobra ion column
source: liquid metal gallium ion source
magnification: 300x to 500kx
probe current: 1 pA to 50 nA
HV: 1.0 to 30 kV
with Pt GIS (gas injection system) to provide both electron and ion beam induced deposition.

Introduction to 3D Reconstruction
Image stack of serial sectioned images at nano-scale can be produced on this system. Using Avizo 3D reconstruction and visualization platform, studies of 3D nano-structure can be achieved.

High Resolution STEM Imaging
Using the retractable BF/DF STEM detector, sample can be imaged in STEM (scanning transmission electron microscopy) mode via electrons have up to 30 kV energy. It provides structure information on a thin specimen.

TEM Sample Preparation
Automatic TEM sample preparation can be done using in-situ lift out method via Kleindiek nano-manipulator (model: MM3A).

Advanced Analytical Platform
A 80 mm2 Oxford SDD (silicon drift detector) EDS system provides a high through-put and high resolution microanalysis capability.

Electron and Ion Beam Lithography
Via Nabity's NPGS system, lithography can be done via either electron or ion beam.

Cryo-capability
A Leica VCT cryo-transfer system is coming soon to provide a cryo-EM capability on this system.

BioScope Resolve's seamless integration of atomic force microscopy and light microscopy reveals more detail and information of biological samples including mechanical properties. The system's unique design makes combined optical and AFM research easy with its open access to the sample, single instrument setup, and correlated data collection and analysis. Researchers can now perfectly connect real-time optical images with AFM imaging.

• The new MIROView graphical interface and ScanAsyst-Cell mode ensure expert data generation, regardless of the user’s AFM experience level.

• Integrates with a Nikon Ti-2 Florescent microscope with:

  • X-cite 120LED Boost light engine

  • 5 filter cubes - BF, DAPI, GFP, DesRed, Cy5

  • 4 Obj lens - 4x, 10x, ELWD20x , and ELWD40x 

  • DIC and BF imaging

• Single, integrated view and operation controls for AFM or optical microscope

• Syncronized AFM images, force maps and single force curves with optical images and data

• Point-and-click setup for automated force and imaging measurements

• Video creation of experiments showing optical and AFM results

• BioAFM accessories and PeakForce Tapping probes to meet every application

• PeakForceQNM mode for accurate mechanical measurement of the materials

Regarding basic information on becoming a self-user, hourly rate, training, etc., please contact Dr. Tsengming (Alex) Chou at tchou@stevens.edu if you have any questions.

Nikon E-1000 up-right florescent microscope with automatic stage control

A high sensitive monochromatic CCD camera for florescent imaging

Nikon C-1 LSCM with 3 lasers

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